1.Think of a good or family member.How woud you describe him or her?
2.what languages do you speak?
3.what do you look like?
4.Do you get nervous when you travel?why or why not?
5.what traditional greetings or activities do you know form other countries?
6.what makes you happy?sad?stressed?
7.what do you do to relax?
Agarose Gel Electrophoresis
1.Mupid II Electrophoresis Apparatus with power supply.
2.Loading dye: 0.25% bromophenol blue ；40%(w/v)sucrose in DDW
3. Autoclaved 50 X TAE buffer:242g Tris base；57.1 ml glacial acetic acid;100ml 0.5M EDTA(pH8.0)；make volume with DDW to 1L
4.Ethedium bromide(EtBr)stock solution:500ug/ml
6.Sterile 1.5-ml eppendorf tubes
7.UV transilluminator box or Image system
1.Prepare 1.2%agarose gel by adding acorrect amount of powered agarose to a measured quantity of 1 X TAE buffer in the glass container.(1.2%agarose gel is suitable for most plasmid DNA electrophoresis.The amount of 1 X TAE buffer added should not be more than 50%volume of the glass container. )
2.Heat the mixture on hot plate or in a microwave oven until the agarose powder dissolves completely.
3.Swirl the mixture and let it cool to abount 60度C before pouring.
4.Set up the casting tray with apporopriare comb.
5.Swirl the agarose solution gently and thoroughly,then pour the warm agarose solution into casting tray.The gel should be between 3 mm and 5 mm thick.
6.After the gel is completely solidifed,remove the comb carefully and lay the gel with gel tray into the electrophoresis tank.
7.Add 1 X TAE buffer enough to cover the gel surface.
8.Mix each DNA samples and the DNA markers with 1 drop of loading dye and load the mixtures into each well slowly and carefully.
9.Put the lid of the gel tank on and attach the electrical cables correctly so that the DNA samples will migrate from cathode(-) toward the anode(+)1 個解答語言1 0 年前