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匿名使用者
匿名使用者 發問時間: 科學其他:科學 · 2 0 年前

為什麼在簡單染色或革蘭氏染色時不易看清萊膜?

為什麼在簡單染色或革蘭氏染色時不易看清萊膜?

我需要淺顯易懂的回答喔~謝謝

3 個解答

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  • 匿名使用者
    2 0 年前
    最佳解答

    莢膜(capsule)是由碳水化合物所組成,且易溶於水,容易在革蘭氏染色時的水洗步驟中被洗掉。

    若使用簡單染色,即是以染劑直接染上整個菌體(不論任何胞器都是全部染上去),無法看清楚菌體的特殊構造。

    莢膜染色則可避這兩個問題。莢膜染色法就是負染法,意即將菌體混在含有染劑的溶液中,染劑會染上背景及菌體,而莢膜可倖免於難。染色結果的透明環,即是莢膜。

    Capsule staining is more difficult than other types of staining procedures because capsule materials are soluble in water and may be dislodged or removed during rigorous washing. To demonstrate the present of capsules, a microbiologist can mix the bacteria in a solution containing a fine colloidal suspension of colored particles ( usually India ink or nigrosin ) to provide a dark background and then stain the bacteria with a simple stain, such as safranin. Because of chemical composition, capsules do not accept most biological dyes, such as safranin, and thus appear as halos surrounding each stained bacterial cell.

    參考資料: Microbiology an introduction, 2002, Tortora, Funke and Case Chapter3 p.71-72
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  • 1 0 年前

    夾膜染色不等於負染法唷!!!

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  • 2 0 年前

    其實並不完全是這樣的...你需要再多查點書

    我懶的打字....哈哈哈

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