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abstract:

The measurement of oxidative stability of beef muscle,from animals fed either control or supplemented vitamin E,during refrigerated storage using both conventional and derivative spectrophotometry was examined.Lipid oxidation measured over a 7-period using both methods was lower in all muscles from animals fed the higher level of supplementation.Area under the curve of MDA-TBA values obtained by derivative analysis were 68to80﹪lower in all muscles than the corresponding TBARS values obtained using conventional spectrophotomerty.Over-all,both methods resulted in similar patterns of oxidation.It

was concluded that both methods were equally as effective in measuring the extent of lipid oxidation in beef muscle during refrigerated display.

Results&Discussion

α-Tocopherol concentration of muscle

The effect of dietary supplementation on the α-Tocopherol concentration of beef SM,ST,andRB can be seen in table1.It is evident that the higher level of supplementation(E3000)resulted in a significantly higherα-Tocopherol concent in all three muscles compared with the corresponding values for the control values.RB had a significantly(P<0.05)higherα-to-copherol content than both SM and ST.No other significant (P>0.05)differences were found

These results are in agreement with previous studies(Arnold and others 1992,1993;Mitsumoto and others 1995;Sanders and other 1997)that reported higherα-Tocopherol concentration in muscle from cattle fed supplementalα-Tocopherol acetate. There are no clear explanations as to why skeletal muscles differ inα-tocophenol concentration(Liu and others 1995).Porter and Palade(1957)reported differences between muscles that contained difference between mucles that contained different proportions of red and white muscle fibers and showed that red fibers contained more but smaller mitochondria than white fibers.Smaller muscle fibers and reduced mitochondrial sizes should,in theory,provide greater surface areas with increased biological membrane volume,thereby providing more interactive sites and membrance surfaces for α-Tocopherol incorporation.Another contributing factor may be the differences in the rate of lipolysis between muscles as reported for chicken thigh(higher)and breast (lower) muscles(De Winne and Dirinck1996).Factors such as rates of oxidative stress,higher concentrations of phospholipids,polyunsaturated fatty acids and prooxidants(iron in particular)in meat may also account for differences in α-Tocopherol levels between different meat types(Wen and others 1997)

1 個解答

評分
  • 2 0 年前
    最佳解答

    難解:

    測定法的氧化的穩定性的牛肉多肌肉部位,從動物餵任一的控制手術室補充維他命E,的時間冷藏庫使用雙方的慣例的和引出的分光光度學被檢查.油脂氧化測量結束7-時期使用雙方的方法是較低的總共肌肉從動物餵較高層次的補充.區域在下曲線的MDA-TBA價值獲得附近引出的分解是68到80﹪較低的總共肌肉比符合的TBARS價值獲得使用慣例的spectrophotomerty.額外的東西-全部,雙方的方法結果是類似的模式的氧化.它

    被推斷雙方的方法是相等地當做有效的在測量的廣度的油脂氧化在牛肉肌肉的時間冷卻陳列.

    結果&討論

    α-生育酚集中的肌肉

    結果的飯食的補充準時地 α-生育酚集中的牛肉機械艙,第,andRB能被在桌子1看.它是明顯的較高層次的補充的( E 3000)結果是意味深長地更高的α-生育酚允許總共3個多肌肉部位比較符合的價值為了控制價值.銣意味深長地有(P<0.05)更高的α-到-copherol滿足比即機械艙又層雲.沒有其他的重要的 (P>0.05)差異被發現

    這些結果是符合先前的學習(安納德等1992,1993;Mitsumoto等1995;紫檀木和其他的1997)那報導更高的α-生育酚集中在肌肉從牛類餵補足的α-生育酚醋酸鹽. 沒有清楚的解釋至於骨骼肌在不同的原因α-tocophenol集中(Liu等1995).看門人和Palade(1957)據報告的差異之間肌肉那個包含差別之間mucles那個包含不同的比例的紅色和白色多肌肉部位光纖和顯示那個紅色光纖包含更多但是小的線粒體比白色光纖.小的多肌肉部位光纖和減少mitochondrial尺寸應該,理論上,提供包括市區及郊區的面積和增加生物膜容量,因此提供更會話式的地點和membrance表面為了 α-生育酚公司.另外的捐贈因素可以是差異在比率的脂解之間肌肉當做報導為了雞大腿(更高的)和乳房 (較低的) 多肌肉部位(判定元件Winne和Dirinck 1996).因素諸如比率的氧化的壓力,更高的集中的磷脂,多元不飽和脂肪酸和prooxidants(燙平特別的)在肉亦可說明差異在 α-生育酚標準之間不同的肉類型(大都市等1997)

    參考資料: 網際護照
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