FelixTung 發問時間: 社會與文化語言 · 1 0 年前

關於生醫的英文翻譯??

Preparation of Tissue

Both eyes were removed from at least two chick embryos on each day between E8 and E21, and placed in phosphate-buffered saline (PBS). The anterior part of the eye ball was discarded. Then after removal of the vitreous from the eye cup, the neural retina was

detached exposing the RPE layer. The eye cups were then fixed in 95% ethanol at 4°C for 30 min, placed in PBS, and their walls consisting of RPE, choroid and sclera were cut into pieces approximately 3 mm square. Four to six tissue pieces from each eye cup

(total of 272 pieces) were prepared and examined.

這一段是在說什麼ㄋ....關於視網膜的....

1 個解答

評分
  • jason
    Lv 5
    1 0 年前
    最佳解答

    組織的準備

    在胚胎的第8天到第21天每天至少要取出兩個胚胎上的兩個眼球,把眼球放在以磷酸緩衝的鹽水溶液(PBS)內。眼球前部棄置不用,眼球後部像個杯狀,就是眼杯部份。

    把眼杯裏面的玻璃體去除,網膜的神經層撥除,這時候底下的網膜色素上皮細胞(retinal pigment epithelium)(RPE)就露出來了。眼杯在4度C下用95%的乙醇固定30分鐘,放入磷酸緩衝液PBS中。這時眼杯壁包含:網膜色素上皮細胞(RPE)、脈絡膜、鞏膜。把眼杯壁切成約3公厘見方的小片。每個眼杯取 4-6 片組織小片,共272片,把這些都準備好開始檢查。

    (譯註:因為共有14天,每天取至少兩個胚胎,每個胚胎有兩個眼睛,每個眼睛切片4-6片,所以共兩百七十多片)

    • Commenter avatar登入以對解答發表意見
還有問題?馬上發問,尋求解答。