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匿名使用者 發問時間: 社會與文化語言 · 1 0 年前

這段英文怎麼那麼難翻譯阿?

好難翻喔!!誰可以幫幫我....(給20點)

A rapid seminested polymerase chain reaction (PCR) method for the specific, sensitive detection of virulent Shigella spp. in spiked environmental water samples was developed. A set of primers specific for the invasion plasmid antigen gene (ipaH) of virulent Shigella spp. and enteroinvasive Escherichia coli produced a 620-bp fragment that was used as template for the seminested primer pair delineating a 401-bp fragment. By using agarose gel electrophoresis for detection of the seminested PCR-amplified products, a detection limit of 1.6×103 cfu S. flexneri was obtained with amplification reactions from crude bacterial lysates. The PCR procedure coupled with an enrichment culture incubated for 6 h detected as few as 1.6 S. flexneri organisms in pure culture. Treated sewage, ground, surface and drinking water samples collected from various sources were seeded with S. flexneri and incubated in GN broth for 6 h before detection by seminested PCR. A detection limit lower than 14 cfu/ml was achieved in some water samples. The results indicate that the described seminested PCR has the advantage of a rapid turnaround time and it fulfills the requirements of sensitivity and specificity for use in an environmental laboratory.

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  • 北北
    Lv 7
    1 0 年前
    最佳解答

    A rapid seminested polymerase chain reaction (PCR) method for the specific, sensitive detection of virulent Shigella spp. 一個迅速的seminested polymerase連鎖回應(PCR)  惡性的Shigella spp的具體,敏感的察覺的方法。  in spiked environmental water samples was developed. 在用大釘釘牢的環境水樣品裡被發展。  A set of primers specific for the invasion plasmid antigen gene (ipaH) of virulent Shigella spp. 一套入門為侵略plasmid抗原基因(ipaH)具體  惡性的Shigella spp。  and enteroinvasive Escherichia coli produced a 620-bp fragment that was used as template for the seminested primer pair delineating a 401-bp fragment. 並且enteroinvasive Escherichia coli 生產為seminested 底漆對刻劃一個401-bp碎片被用作模板的一個620-bp碎片。  By using agarose gel electrophoresis for detection of the seminested PCR-amplified products, a detection limit of 1.6×103 cfu S. 透過seminested PCR 放大的產品,1.6  03 cfu S的檢測極限的察覺使用agarose 凝膠電泳。  flexneri was obtained with amplification reactions from crude bacterial lysates. flexneri被來自天然細菌的lysates的擴大回應獲得。  The PCR procedure coupled with an enrichment culture incubated for 6 h detected as few as 1.6 S. PCR 程式加上一種富裕文化為發現象2世紀6年代一樣少的6 h孵化。  flexneri organisms in pure culture.在純文化裡的flexneri 組織。  Treated sewage, ground, surface and drinking water samples collected from various sources were seeded with S.  從各種各樣的來源收集的處理的污水,地,表面和飲用水樣品被S結子。  flexneri and incubated in GN broth for 6 h before detection by seminested PCR.  flexneri和在seminested PCR的察覺之前為6 h在GN 肉湯裡孵化。  A detection limit lower than 14 cfu/ml was achieved in some water samples. 比14 cfu/ml 低的檢測極限被在一些水樣品內取得。  The results indicate that the described seminested PCR has the advantage of a rapid turnaround time and it fulfills the requirements of sensitivity and specificity for use in an environmental laboratory. 結果表明被描述的seminested PCR有迅速的周轉時間方面的優勢, 它滿足敏感性和特異性的要求供一間環境實驗室使用。 

  • 1 0 年前

    迅速seminested polymerase 鏈式反應(PCR) 方法為劇毒Shigella 的具體, 敏感偵查spp. 在尖環境水樣被開發了。一套底漆具體為入侵質粒抗原基因(ipaH) 劇毒Shigella spp. 和enteroinvasive 大腸埃希氏菌導致了被使用的一個620 bp 片段當模板為seminested 更加雷管的對描述401 bp 片段。由使用agarose 膠凝體電泳法為偵查seminested PCR 被放大的產品, 1.6□03 cfu S. flexneri 檢測極限被獲得了以放大作用反應從粗暴細菌lysates 。PCR 做法被結合與充實文化孵化了為6 h 查出只有1.6 S. flexneri 有機體在純文化。被對待的汙水、地面、表面和飲用水樣品從各種各樣的來源被收集播種了與S. flexneri 和被孵化了在GN 湯裡為6 h 在偵查seminested PCR 之前。檢測極限更低比14 cfu/ml 達到了在一些水樣。結果表明, 被描述seminested PCR 有迅速歸航時間的好處並且它履行敏感性和特異性的要求用於一個環境實驗室。

    參考資料: 自己跟朋友
  • 1 0 年前

    你這翻譯!是翻譯軟體的!翻起來超不順的說...

    2005-11-22 21:23:29 補充:

    S.flexneri 是個微生物.S=SHGELLA

  • 1 0 年前

    迅速seminested polymerase 鏈子反應(PCR) 方法為具體, 敏感偵查劇毒 Shigella spp 。 在尖環境水樣被開發了。 套底漆具體為入侵質粒抗原基因(ipaH) 劇毒 Shigella spp 。 並且enteroinvasive 大腸埃希氏菌導致了一個620 bp 片段那被使用了當模板為seminested 更加雷管的對描述401 bp 片段。 由使用agarose 形成膠凍電泳法為偵查seminested PCR 被放大的產品, 檢測極限1 。6.6N103 cfu S 。 flexneri 是獲得以放大作用反應從粗暴細菌lysates 。 PCR 做法被結合與充實文化孵化了為6 h 被查出作為少數作為1 。6 S 。 flexneri 有機體在純文化。 被對待的汙水, 地面, 表面並且飲用水樣品從各種各樣的來源被收集播種了以S 。 flexneri 和孵化在GN 湯裡為6 h 在偵查之前seminested PCR 。檢測極限更低比14 cfu/ml 達到了在一些水樣。 結果表明, 被描述seminested PCR 有好處迅速歸航時間和它履行敏感性和特異性的要求用於一個環境實驗室。

    應該是這樣ㄅ~

    參考資料: 自己
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