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B. thuringiensis was isolated from two commercially

available insecticides, DipelRDF (Abbott Laboratories,

Chicago, USA) and DelfinRWG (Thermo

Trilogy, Columbia, USA), that use this organism as

the active constituent. Insecticide (1 g) was suspended

in 9 ml of 0.1% bacteriological peptone

(Oxoid, Melbourne, Australia), diluted, spread inoculated

on plates of tryptone soya agar (TSA, Oxoid)

and incubated at 30 jC for 24 h. Colonies were

isolated and purified by streaking. These strains

were maintained on slants of nutrient agar (NA,

Oxoid), after incubating at 30 jC for 24 h. Two

strains of O. oeni were similarly isolated by plating

samples from commercial starter culture products

onto MRSA agar. MRSA agar consisted of de

Man, Rogosa and Sharpe agar (MRS, Oxoid) supplemented

with 15% (v/v) apple juice (Berri, Australia)

and adjusted to pH 5.5 using HCl. Plates were

incubated at 25 jC for 7 days. O. oeni 1 was

isolated from Viniflora CH35k and O. oeni 2 was isolated from Viniflora

Oenosk . Other cultures of bacteria,

yeasts and filamentous fungi used in the study are

listed in Table 1 and were obtained from collections

within the University of New South Wales (UNSW),

the Australian Wine Research Institute (AWRI) and

Food Science Australia, CSIRO. Strains of Aureobasidium

pullulans, Cryptococcus species, Metschnikowia

pulcherrima, Rhodotorula sloofiae and

Sporobolomyces roseus were originally isolated from

wine grapes cultivated in the Hunter Valley region,

New South Wales.

2.2. Grape samples

Grapes samples, collected during October–February

were obtained from eight commercial vineyards

located in the upper and lower Hunter Valley, Mudgee

and Griffith regions of New South Wales,

Australia. Grapes were sampled at several phenological

stages of development, 大意就可

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    1 0 年前
    最佳解答

    B。 thuringiensis 分開二商業地

    可得的殺蟲劑, DipelRDF (Abbott 實驗室,

    芝加哥,美國)和 DelfinRWG (Thermo

    三部劇,哥倫比亞,美國), 那一種使用這一個生物當做積極的成份。 殺蟲劑 (1 g)是中止的

    在 0.1% 細菌學的消化蛋白質的 9 毫升中

    (Oxoid ,墨爾本,澳洲), 沖淡,傳佈接種

    在 tryptone 大豆瓊脂的碟子上(避稅年金, Oxoid)而且為 24 h 以 30 jC 抱。 殖民地是

    藉著裸奔隔離而且潔淨。 這些緊張在營養瓊脂的傾斜上被維護(NA,Oxoid), 在為 24 h 以 30 jC 抱之後。 二

    O 的緊張。 oeni 同樣地被電鍍隔離了

    來自商業的起動器文化產品的樣本

    在 MRSA 瓊脂之上。 MRSA 瓊脂有 de

    男人, Rogosa 和 Sharpe 瓊脂 (太太 , Oxoid)補充

    藉由 15%(v/v)蘋果擠出汁來(Berri 澳洲)

    而且調整到使用 HCl 的 pH 5.5。 碟子是

    以 25 jC 抱 7 天。 O。 oeni 1 是

    從 Viniflora CH35 k 和 O 隔離。 oeni 2 與 Viniflora 分開

    Oenosk 。 其他細菌的文化,

    被用於研究的酵母和細絲狀的真菌是

    在表 1 中列出而且從收集被獲得

    在新威爾斯 (UNSW)南部的大學裡面,

    澳洲葡萄酒研究學會 (AWRI)和

    食物科學澳洲, CSIRO。 Aureobasidium 的緊張

    pullulans , Cryptococcus 品種, Metschnikowia

    pulcherrima , Rhodotorula sloofiae 和

    Sporobolomyces roseus 本來分開

    葡萄酒葡萄在獵人山谷區域中種植,

    新的南方條痕

    2.2.葡萄抽樣調查

    葡萄樣本,在十月-二月期間收集

    從八個商業的葡萄園被獲得

    在上面的和比較低的獵人山谷設置, Mudgee

    而且新威爾斯南部的格里菲斯區域,

    澳洲。 葡萄在一些 phenological 被抽樣調查

    發展的階段,

    大概是這樣吧!!

    參考資料: 我..和字典
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