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The first protein that promotes microtubule catastrophes was isolated Xenopus egg extracts by a group of researchers who were searching for proteins related to kinesin,a motor protein thought to be involved in spindle assembly. Using an antibody that recognizes kinesins,the researchers isolated a protein that is required for normal spindle assembly in Xenopus egg extracts.

The protein,which they dubbed XKCM1 (for Xenopus kinesin central motor 1), affected microtubule dynamics quite dramatically. When the protein was removed from a mitotic extract using anti-kinesin antibodies, the resuting spindls contained abnormally long microtubules, similar in length to the microtubules formed in interphase exracts. This chang in spindle formation was caused specifically by depletion of XKCM1, as when the pro-tein was added back to the depleted extracts, the micro-tubule asters returned to their normal size. furthermore,the researchers demonstrated that XKCM1 promotes micro-tubule catastrophes in Xenopus extracts: depletion of the protein reduced the frequency of catastrophes, allowing the mitotic microtubles to grow to extreme lengths. Such pro-teins are now called catastrophins.

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  • 1 0 年前
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    促進microtubule 浩劫的第一蛋白質是被隔絕的Xenopus 蛋萃取物由尋找蛋白質與kinesin 有關的一個小組研究員, 馬達蛋白質被認為被介入在紡錘彙編裡。使用認可kinesins 的抗體, 研究員隔絕了必需為正常紡錘彙編在Xenopus 蛋萃取物裡的蛋白質。蛋白質, 他們叫XKCM1 (為Xenopus kinesin 中央馬達1), 受影響的microtubule 動力學相當顯著。當蛋白質從一種mitotic 萃取物被取消了使用anti-kinesin 抗體, resuting 的spindls 包含了反常地長的microtubules, 相似在長度與microtubules 被形成在相界面exracts 。這個chang 在紡錘形成由XKCM1 的取盡具體地造成, 和當蛋白質增加了回到被耗盡的萃取物, micro-tubule 翠菊返回到他們的正常大小此外, 研究員顯示出, XKCM1 促進micro-tubule 浩劫在Xenopus 萃取物裡: 蛋白質的取盡減少了浩劫頻率, 允許mitotic microtubles 成長為極端長度。這樣蛋白質現在叫做catastrophins 。

    參考資料: 奇摩自典
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