Restriction Enzyme Action of EcoRI
The EcoRI restriction enzyme--the first restriction enzyme isolated from E. Coli bacteria--is able to recognize the base sequence 5' GAATTC 3'. Restriction enzymes cut each strand of DNA between the G and the A in this sequence. This leaves "sticky ends" or single stranded overhangs of DNA. Each single stranded overhang has the sequence 5" AATT 3'. These overhanging ends will bond to a fragment of DNA which has the complementary sequence of bases. See text of Background Paper for additional details.
能認可基本的序列5' GAATTC 3' ,限制酵素切開DNA 兩股之間在這個序列在G 和A 。
就是要兩個條件 第一個要在5' GAATTC 3' 第二個 在浙東間的 GA會被切開