The fasting blood glucose concentration was determined using MediSense Card Blood Glucose Testing Systems , which uses an electrochemical detection technique with a glucose oxidase method. One day before the fasting blood glucose levels were measured, the postprandial urinary levels of ketone bodies, glucose and protein, the pH values, and the presence of blood were determined using multiple test strips . Complete blood counts, including the levels of red blood cells, white blood cells, hemoglobin, hematocrit, and platelet, were measured using a hematology analyzer . Serum concentrations of albumin, cholesterol, triglyceride, creatinine, blood urea nitrogen (BUN), and uric acid were measured by an automatic analyzer .
Serum concentrations of free fatty acid and fructosamine were determined by enzymatic colorimetric assays , and the insulin concentrations in the serum and pancreas were measured by commercially available enzyme-linked immunosorbant assays . The pancreatic insulin was extracted by hydrochloride-acidified ethanol, as described by Masiello et al, prior to measuring the insulin. All the samples were analyzed in one assay in duplicate with intra-assay coefficients of variation below 10%.
Animal carcass compositions, such as percentage and amount of water, protein, and fat, were measured with methods as previously described . In brief, total carcass water was determined by freeze-drying the carcasses for 2 weeks to obtain a dried carcass weight and then subtracting the dried carcass weight from the total carcass weight. The freeze-dried carcasses were then homogenized with liquid nitrogen, and aliquots of dried rat homogenate were assayed for nitrogen and fat concentrations. The carcass fat concentration was determined by extracting dried rat homogenate with ether, and then evaporating the ether to determine the fat amount. In addition, carcass nitrogen concentration was determined by micro-Kjeldahl analysis.
- 嵐兮Lv 51 0 年前最佳解答
（還真是挺難翻的）空腹血糖值是用MediSense卡片血糖測試系統來偵測的，這個系統以葡萄糖氧化酶法應用電化學偵測技術。將來有一天，在空腹血糖被測量之前，飯後尿液酮體、葡萄糖、蛋白質、酸鹼度、以及血尿可利用多重試驗試紙來偵測。全血細胞計數， 包括紅血球、白血球、血紅素、血比容、以及血小板則使用血液學分析儀測量。 至於血清白蛋白、膽固醇、三酸甘油酯、肌酸酐、血液尿素氮、以及尿酸，則以自動化分析儀來分析。血清游離脂肪酸和果糖胺以酶比色法分析、血清胰島素與胰臟內胰島素濃度以可購買到的「酵素鏈接免疫吸附分析法」（即ELISA法）分析。胰臟內胰島素先以鹽酸酸化酒精提取（如Masiello等所描述），再進行測量。所有樣本在同一分析中均以一式兩份來進行，兩個分析結果變化參數需小於10%（意指同一樣本兩次分析誤差不超過10%） 動物體內的水份、蛋白質、脂肪的含量以早先描述的方法測量。簡而言之，動物體內含水量是以動物體總重量減去冰凍乾燥兩個星期的動物體乾體重來計算。被冰凍乾燥的動物體先和液態氮均勻混合，再進行含氮量和脂肪含量檢驗。先以乙醚萃取乾燥鼠體的脂肪，然後將乙醚汽化以定量動物體脂肪含量。另外，動物體氮含量係以於micro-Kjeldahl 法分析。