徵求英文「翻譯」英文期刊(急需~~20點)!!!!!

Human subject approval from the University of Michigan was obtained to use residual amniocentesis samples that were to be discarded. To be eligible for the study, pregnant women had to be undergoing clinically indicated amniocentesis and have intact membranes. The only information on the women available to the investigators was age, race/ethnicity, and number of fetuses present at time of sampling. All clinical assays related to chromosomal assessment of the fetus had been completed, and the residual amniotic fluid and amniotic cell culture pellets were retrieved from the cytogenetics laboratory.

Amniotic fluid samples (8–12 ml) were spun at 4500 rpm for 30 minutes. After decanting the supernatant fluid, the pellet was suspended in 150 μL of TrisEDTA buffer. The cultured cell pellets were resuspended in 200 μL PBS. A 150 μL volume of each sample was extracted using the Roche MagNA Pure LC instrument and the DNA isolation kit I resulting in a final volume of 150 μL.

We used a direct PCR method followed by a nested PCR method to detect HPV. The Roche line blot assay, based on L1 consensus PCR with biotinylated PGMY09/11 primer sets and β-globin as an internal control for sample amplification [17,18] was used as previously described, with 10 μL extract in each 50 μL reaction. All samples were HPV gel-band-negative and Roche Prototype Strip Assay-negative after 40 cycles (reagents provided as a gift from Roche Molecular Systems, Inc., Pleasanton, CA).

For the nested reaction, five microliters of each L1 amplicon was added to a PCR reaction mix containing GP5+/GP6+ [19] primers (1 μM each) and run for an additional 40 cycles under the following conditions: 40 cycles of 94°C for 45 s, 48°C for 4 s, 38°C for 30 s, 42°C for 5 s, 66°C for 5 s, and 71°C for 1.5 min. This was followed by a final extension of 10 min at 72°C. Fifteen μL from each sample was analyzed on a 2% agarose gel.

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請不要用YAHOO翻譯

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不過也謝謝喔

2 個解答

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  • 1 0 年前
    最佳解答

    Human subject approval from the University of Michigan was obtained to use residual amniocentesis samples that were to be discarded. 人類絨毛膜細胞檢體乃由密西根州立醫院(人體試驗委員會)認可合法取得,剩餘檢體均依法丟棄。To be eligible for the study, pregnant women had to be undergoing clinically indicated amniocentesis and have intact membranes. 為了實驗的正確性,參與本研究的產婦都必需有完整的絨毛膜以供穿刺The only information on the women available to the investigators was age, race/ethnicity, and number of fetuses present at time of sampling. 研究者只知道產婦的年齡、種族和前胎個數(生過幾個)。All clinical assays related to chromosomal assessment of the fetus had been completed, and the residual amniotic fluid and amniotic cell culture pellets were retrieved from the cytogenetics laboratory.

    所有的絨毛膜細胞檢體都已經做過染色體檢查才從細胞遺傳室拿出來分離培養的。檢體包括有絨毛膜組織及絨毛液。Amniotic fluid samples (8–12 ml) were spun at 4500 rpm for 30 minutes.每12CC 絨毛液以4500 rpm離心30分鐘

    After decanting the supernatant fluid, the pellet was suspended in 150 μL of TrisEDTA buffer.倒掉上清液後以150μL TrisEDTA buffer. 再懸浮 The cultured cell pellets were resuspended in 200 μL PBS.若是拿到的是遺傳室剩下細胞培養過的檢體 同樣以以4500 rpm離心30分鐘 然後再懸浮於200 μL PBS中 A 150 μL volume of each sample was extracted using the Roche MagNA Pure LC instrument and the DNA isolation kit I resulting in a final volume of 150 μL.上述的兩種檢體均以羅氏全自動DNA萃取機 MagNA Pure LC 進行基因純化,最後得到150 μL的DNA。

    We used a direct PCR method followed by a nested PCR method to detect HPV.

    我們用direct PCR 先將基因放大 然後用 nested PCR 進行HPV(人類乳突狀病毒)進行分型。

    2006-11-28 23:52:10 補充:

    在40個cycle後 所有檢體無論是跑膠或是用Prototype Strip Assay 都是呈現陰性為了證明陰性的正確性 我門又用了三條Primer GP5+/GP6+ [19] primers跑40 cycles .最後以72°C 10 min extension 跑2%膠

    2006-11-28 23:53:19 補充:

    翻譯費700

    2006-11-28 23:54:02 補充:

    可用2億太子幣還我

  • 1 0 年前

    人類主題認同從密執安大學被獲得使用將被擯除的殘餘的羊膜穿刺術樣品。是有資袼研究, 孕婦必須接受臨床被表明的羊膜穿刺術和有原封膜。唯一的資訊關於婦女可利用對調查員是年齡、胎兒的race/ethnicity, 和數字當前在採樣的時期。所有臨床分析用試樣與對胎兒的染色體評估關係了被完成了, 並且殘餘的羊膜流體和羊膜細胞培養藥丸被檢索了從細胞遺傳學實驗室。羊膜流體樣品(8-12 機器語言) 轉動了在4500 轉每分鐘30 分鐘。在傾析浮在表層的流體以後, 藥丸暫停了在150 TrisEDTA 緩衝?L 。被開化的細胞藥丸被重新懸掛了在200?L PBS 。各個樣品的150?L 容量被提取了使用Roche 優秀大學畢業生純淨的LC儀器和DNA 隔離成套工具I 造成150 的最後的容量?L 。我們使用了一個直接PCR 方法被一個被築巢的PCR 方法跟隨查出HPV 。Roche 線汙點分析用試樣, 根據L1 公眾輿論PCR 與biotinylated PGMY09/11 底漆集合和?-globin 如同內部控制為樣品放大作用[ 17,18 ] 依照早先被描述被使用了, 以10?L 萃取物在各50?L 反應。所有樣品是HPV 膠凝體帶消極和Roche 原型小條分析用試樣消極在40 個週期(試劑以後被提供作為一件禮物從Roche Molecular Systems, Inc., Pleasanton, 加州) 。為被築巢的反應, 五微升各L1 amplicon 增加了來PCR反應混合包含GP5+/GP6+ [ 19 ] 底漆(1?M 每個) 並且奔跑為另外的40 個週期在以下條件下: 94.C 的40個週期為45 s 、48.C 為4 s, 38.C 為30 s, 42.C 為5 s, 66.C 為5 s, 和71.C 1.5 分鐘。這被10 分鐘一個最後的引伸跟隨了在72.C 。十五?L 從各個樣品被分析了在2% agarose 膠凝體。

    參考資料: 自己跟老師翻的...
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