Ken 發問時間: 社會與文化語言 · 1 0 年前

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Molecular-beacon-based array for sensitive DNA analysis

Molecular beacon (MB) DNA probes provide a new way for

sensitive label-free DNA/ protein detection in homogeneous solution

and biosensor development.

However, a relatively low fluorescence enhancement after the

hybridization of the surface-immobilized MB hinders its effective

biotechnological applications.

We have designed new molecular beacon probes to enable a larger

separation between the surface and the surface-bound MBs.

Using these MB probes, we have developed a DNA array on

avidin-coated cover slips and have improved analytical sensitivity.

A home-built wide-field optical setup was used for imaging the array.

Our results show that linker length, pH , and ionic strength have

obvious effects on the performance of the surface-bound MBs.

The fluorescence enhancement of the new MBs after hybridization

has been increased from 2 to 5.5.

The MB-based DNA array could be used for DNA detection with

high sensitivity, enabling simultaneous multiple-target bioanalysis in

a variety of biotechnological application.

3 個解答

評分
  • 1 0 年前
    最佳解答

    為敏感的 DNA 分析的分子-位於信號的排列

    Molecular-beacon-based array for sensitive DNA analysis

    Molecular beacon (MB) DNA probes provide a new way for

    sensitive label-free DNA/ protein detection in homogeneous solution

    and biosensor development.

    However, a relatively low fluorescence enhancement after the

    hybridization of the surface-immobilized MB hinders its effective

    biotechnological applications.

    We have designed new molecular beacon probes to enable a larger

    separation between the surface and the surface-bound MBs.

    Using these MB probes, we have developed a DNA array on

    avidin-coated cover slips and have improved analytical sensitivity.

    A home-built wide-field optical setup was used for imaging the array.

    Our results show that linker length, pH , and ionic strength have

    obvious effects on the performance of the surface-bound MBs.

    The fluorescence enhancement of the new MBs after hybridization

    has been increased from 2 to 5.5.

    The MB-based DNA array could be used for DNA detection with

    high sensitivity, enabling simultaneous multiple-target bioanalysis in

    a variety of biotechnological application.

    分子的信號 (MB) DNA 調查提供一個新的方法給

    同種的解決方案的敏感的無標籤 DNA/蛋白質發現

    而且 biosensor 發展。

    然而,相對低的螢光提高在那之後

    表面使不動 MB 的雜交阻礙它的有效的

    biotechnological 申請。

    我們已經設計新的分子信號調查能夠一比較大的

    表面和表面之間的分離-bo

    參考資料: 朋友的解答.希望對你有幫助^^
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  • 1 0 年前

    看的懂也*的..不怎中文的中文

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  • 1 0 年前

    基於分子烽火臺的列陣為敏感DNA 分析

    分子烽火臺(MB) DNA 探針為敏感無標記DNA/ 蛋白質偵查提供一個新方式在同類的解答和生理傳感器發展。

    但是, 一種相對地低熒光改進在表面被固定的MB 的雜交以後妨害它有效的biotechnological 應用。

    我們設計新分子烽火臺探針使能更大的分離在表面之間並且表面跳起MBs 。

    使用這些MB 探針, 我們開發了一個DNA 列陣在抗生物素蛋白上漆的蓋子滑動和改進了分析敏感性。

    一個家被修造的寬領域光學設定被使用了為想像列陣。

    我們的結果表示, 連接器長度、酸鹼度, 和離子強度有明顯的作用在表現表面跳起MBs 。

    新MBs 的熒光改進在雜交被增加了從2 到5.5 以後。

    基於MB 的DNA 列陣能被使用為DNA 偵查以高敏感性, 使能同時多目標bioanalysis 在各種各樣biotechnological 應用。

    如有錯誤請多多指教。

    參考資料: 英文字典
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