一隻豬 發問時間: 社會與文化語言 · 1 0 年前

急急急..救命啊..麻煩中翻英(不要翻譯軟體的) 拜託ㄟ

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2001年在屏東田間發現沙漠玫瑰(Adenium obesum)疑似感染病毒病,經前人研究,分離出直徑約28-30nm之球型病毒。本研究之目的即在進一步探討此一病毒之純化及組織病理學。以接種病毒之台煙五號菸草病葉為材料,經高低速離心及蔗糖密度梯度離心,可獲得純化之球形病毒,其100克台煙五號病葉之病毒收量為1.44mg。取田間沙漠玫瑰嵌紋病葉及接種沙漠玫瑰病毒之台煙五號病葉經包埋處理後做超薄切片,可觀察到田間沙漠玫瑰植物細胞內細胞壁變形、病毒粒子於細胞內聚集及病毒在細胞內造成之空泡;接種沙漠玫瑰病毒之台煙五號菸草細胞內亦可觀察到病毒粒子聚集、葉綠餅被分解及病毒粒子聚集而成之結晶。瓊脂雙向擴散反應,結果顯示此沙漠玫瑰所分離出之球型病毒會與胡瓜嵌紋病毒(Cucumber mosaic virus(CMV))抗血清發生專一性沉澱反應。病毒鞘蛋白經SDS-PAGE分析估算分子量為26KDa。間接式酵素連結免疫分析結果顯示,嘉義田間沙漠玫瑰嵌紋病與CMV抗血清有正反應。

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  • 慧宣
    Lv 5
    1 0 年前
    最佳解答

    Discover desert's rose in 2001 between Ping tung's field (Adenium obesum) doubt be like infection virus disease, by forefathers research, separate come out diameter about 28-30nm zhi ball-type virus. What this research's aim that be at furthur inquire into this a virus zhi purify and organization pathology. As vaccinate virus zhi platform's smoke's five number yans grass illness leaf for material, by discretion quick centrifugal and saccharose's density gradient centrifugal, can acquire purify zhi sphericity's virus, its 100 grammes platform smokes five No. illness leaf zhi virus receive quantity for 1.44mg. Take desert's rose iframe lines illness leaf and vaccinate desert's rose's virus zhi platform's smoke's five No.s illness leaves by wrap bury dispose behind make very thin slice between field, can observe go desert's rose's plant's cell in cell wall distortion between field, virus' particle in assemble and virus at cell in result in zhi empty bubble in cell; vaccinate may also observe go virus particle assemble in desert's rose's virus zhi platform's smoke's five number yans grass cell, leaf green cake cover analyse and virus particle assemble but become zhi crystal. Agar-agar's bilateral diffusion's reaction, kill show this' desert's rose separate come out zhi ball-type virus can with cucumber iframe lines virus (Cucumber mosaic virus (CMV))resist blood serum take place specificity sediment's reaction. Virus qiao albumen by SDS- PAGE analyze estimate molecular weight for 26KDa. Indirect style ferment conjoin immunization analysis' result show, Chiayi's tian jian's desert rose iframe lines illness with CMV resist blood serum have plus reaction.

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