Polyphenolic compounds exert a variety of physiological effects in vitro including antioxidative, immunomodulatory and antigenotoxic effects. In a randomized crossover study in healthy men on a low-polyphenol diet, we determined the effects of 2 polyphenol-rich juices (330 ml/d) supplemented for 2 weeks on bioavailability of polyphenols, markers of antioxidative and immune status, and reduction of DNA damage. Juices provided 236 mg (A) and 226 mg (B) polyphenols with cyanidin glycosides (A) and epigallocatechin gallate (B) as major polyphenolic ingredients. There was no accumulation of plasma polyphenols after two weeks of juice supplementation. In contrast, plasma malondialdehyde decreased with time during juice interventions. Moreover, juice consumption also increased lymphocyte proliferative responsiveness, with no difference between the two juices. Interleukin-2 secretion by activated lymphocytes and the lytic activity of natural killer cells were significantly increased by both juices. Juice intervention had no effect on single DNA strand breaks, but significantly reduced oxidative DNA damage in lymphocytes. A time-delay was observed between the intake of fruit juice and the reduction of oxidative DNA damage and the increase in interleukin-2 secretion. We conclude that consumption of either juice enhanced antioxidant status, reduced oxidative DNA damage and stimulated immune cell functions. However, fruit juice consumption for 2 weeks did not result in elevated plasma polyphenols in subjects after overnight fasting. Further studies should focus on the time-delay between juice intake and changes in measured physiological functions, as well as on active polyphenolic metabolites mediating the observed effects.
- 1 0 年前最佳解答
Polyphenolic 化合物施加各種各樣的生理作用在試管內包括抗氧化, 免疫調節和antigenotoxic 作用。在一項被隨機化的天橋研究中在健康人在低多酚飲食, 我們確定了2 富有多酚的汁液的作用(330 ml/d) 被補充2 個星期在多酚、DNA 損傷的標誌抗氧化和免疫狀態, 和減少的生物相容性。汁液提供236 毫克(a) 和226 毫克(b) 多酚以cyanidin glycosides (a) 和epigallocatechin 沒食子酸鹽(b) 作為主要polyphenolic 成份。沒有血漿多酚的儲積在二個星期汁液補充以後。相反, 血漿malondialdehyde 減少了以時間在汁液干預期間。而且, 汁液消耗量並且增加了淋巴細胞proliferative 快速響應, 沒有區別在二汁液之間。Interleukin-2 分泌物由被激活的淋巴細胞和NK 細胞的細胞溶解的活動被兩汁液顯著增加了。汁液干預沒有對唯一DNA 子線斷裂的作用, 而是顯著被減少的氧化DNA 損傷在淋巴細胞。time-delay 被觀察了在果汁和氧化DNA 損傷的減少進水閘和在interleukin-2 分泌物的增量之間。我們認為, 或者汁液的消耗量提高了抗氧化狀態、被減少的氧化DNA 損傷和被刺激的免疫細胞作用。但是, 果汁消耗量2 個星期沒有導致被舉起的血漿多酚在主題在隔夜齋戒以後。進一步研究應該集中於time-delay 在汁液進水閘和變化之間在被測量的生理作用上, 並且在活躍polyphenolic 代謝產物斡旋被觀察的作用。 翻譯
- 匿名使用者5 年前