celine 發問時間: 社會與文化語言 · 1 0 年前

有關HPLC的英文檔案,可以幫我翻譯麼

HPLC stability-indicating method was validated for Vitamin C (ascorbic acid) in semisolid pharmaceutical/cosmetic formulations containing glutathione and sodium metabisulfite, as antioxidants. The described procedure included a reliable, precise, accurate and specific method determination employing a 250 mm × 4.6 mm C18 column, 0.2% metaphosphoric acid/methanol/acetonitrile (90:8:2, v/v/v) as the mobile phase and detection at 254 nm. Nicotinic and ascorbic acids were employed as standards, both presenting purity of 99.0%. Linearity was established for the ascorbic acid concentrations ranging form 1.0 to 12 μg mL−1, accuracy/recovery percentage was 95.46–101.54%, precision values were 0.38 (intra-day) and 1.22% (inter-days), and LOD and LOQ were found to be 0.05 and 0.17 μg mL−1, respectively. The working mobile phase elevated the ascorbic acid retention time to ≈3.5 min at a flow rate of 1.0 mL min−1 and provided resolution of the active from the nicotinic acid (internal standard), degradation product (oxalic acid) and other excipients from the pharmaceutical/cosmetic preparations.

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  • 1 0 年前
    最佳解答

    HPLC 穩定表明的方法被確認了為維生素C (抗壞血酸) 在半固體pharmaceutical/cosmetic 公式化包含glutathione 和鈉metabisulfite, 作為抗氧劑。被描述的做法包括可靠, 精確, 準確和具體方法決心使用一個250 毫米□4.6 毫米C18 專欄, 0.2% metaphosphoric acid/methanol/acetonitrile (90:8:2, v/v/v) 作為流動相和偵查在254 毫微米。尼古丁和抗壞血酸被使用了作為標準, 兩個提出純淨99.0% 。線性為抗壞血酸含量排列的形式1.0 建立了到12?g mL?1, accuracy/recovery 百分比是95.46-101.54%, 精確度價值是0.38 (內部天) 並且1.22% (相互天), 並且LOD 並且LOQ 被發現0.05 和0.17?g mL?1, 各自地。工作流動相被舉起抗壞血酸保留時間對?3.5 分鐘以1.0 機器語言的流速min?1 和假設激活的決議從煙酸(內部標準), 退化產品(草酸) 並且其它賦形劑從pharmaceutical/cosmetic 準備。

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  • 1 0 年前

    HPLC 穩定表明的方法被確認了為維生素C (抗壞血酸) 在半固體pharmaceutical/cosmetic 公式化包含glutathione 和鈉metabisulfite, 作為抗氧劑。被描述的做法包括可靠, 精確, 準確和具體方法決心使用一個250 毫米□4.6 毫米C18 專欄, 0.2% metaphosphoric acid/methanol/acetonitrile (90:8:2, v/v/v) 作為流動相和偵查在254 毫微米。尼古丁和抗壞血酸被使用了作為標準, 兩個提出純淨99.0% 。線性為抗壞血酸含量排列的形式1.0 建立了到12?g mL?1, accuracy/recovery 百分比是95.46-101.54%, 精確度價值是0.38 (內部天) 並且1.22% (相互天), 並且LOD 並且LOQ 被發現0.05 和0.17?g mL?1, 各自地。工作流動相被舉起抗壞血酸保留時間對?3.5 分鐘以1.0 機器語言的流速min?1 和假設激活的決議從煙酸(內部標準), 退化產品(草酸) 並且其它賦形劑從pharmaceutical/cosmetic 準備。

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