發問時間: 社會與文化語言 · 1 0 年前

超急英文翻成中文

2. Methods and materials

2.1. Animals and diet

Male albino Wistar rats (body weight, 170-200 g) were

obtained from the Central Animal House, Rajah Muthiah

Medical College, Annamalainagar, India, and were maintained

under a constant 12-hour light-dark cycle at 218C to

238C. The animals were maintained in accordance with the

guidelines of the National Institute of Nutrition, Indian

Council of Medical Research, Hyderabad, India. The study

was approved by the institutional ethical committee,

Bharathidasan University, Tamil Nadu, India. Throughout

the experimental period, the rats were fed a balanced

commercial pellet diet (Hindustan Lever Ltd, Mumbai,

India) with a composition of 5% fat, 21% protein, 55%

nitrogen-free extract, and 4% fiber (wt/wt) with adequate

mineral and vitamin levels for the rat. Diet and water were

provided ad libitum.

2.2. Drugs and chemicals

All the chemicals used in this experiment were obtained

from Sigma Chemical Company (St Louis, Mo), Hi Media

(Mumbai, India), or SD-Fine Chemicals (Mumbai, India).

All chemicals used were of analytical grade.

2.3. Plant material

G montanum leaves were collected from the Shola forests

of the Nilgiri Biosphere Reserve in Western Ghats, Gudalur,

at an elevation of 900 to 1500 m above sea level. The plant

was identified at the Herbarium of Botanical Survey of

India, Southern Circle, Coimbatore, India (accession no.

32561-65) and was deposited in the Department of Botany,

Bharathiar University, Coimbatore, India.

2.4. Preparation of GLEt

To prepare the extract (GLEt), 500 g of fresh leaves of

G montanum was chopped into small pieces and soaked

overnight in 1.5 L of 95% ethanol. This suspension was

filtered, and the residue was resuspended in an equal

volume of 95% ethanol for 48 hours and filtered. The 2

filtrates were pooled, and the solvent was evaporated in a

rotavapor at 408C to 508C under reduced pressure. The

greenish-black residue (6 25 g) was lyophilized and

stored at 08C to 48C until further use. When needed,

the residue was suspended in distilled water and used in

the study.

1 個解答

評分
  • 東東
    Lv 4
    1 0 年前
    最佳解答

    albino Wistar公鼠(約170-200克重)來自印度Annamalainagar Rajah Muthiah 醫學大學內中央動物學院,會以12小時的日夜循環和21.8C至23.8C之間養育。

    照料環境和位於印度Hyderabad的Indian Council of Medical Research內國家營養中心的標準一致。

    本研究由位於印度Tamil Nadu的Bharathidasan大學內的學術倫理委員會所核可。

    在實驗階段,老鼠以均衡的商業用飼料(Hindustan Lever Ltd, Mumbai, India)餵食,其中含有5%脂肪、21%蛋白質、55%不含氮的抽取物以及4%的纖維,此外還有足量的礦物質和維生素。

    而飲食則是隨意地由老鼠取用。

    本研究中所用到的化學物質是由以下三個機構中取得:Sigma Chemical Company (St Louis, Mo), Hi Media (Mumbai, India), or SD-Fine Chemicals (Mumbai, India)。

    所有的化學物質都在分析等級。

    G montanum是在Shola forests of the Nilgiri Biosphere Reserve in Western Ghats, Gudalur當地,海拔900-1500公尺處所取得。

    G montanum隨後送至Herbarium of Botanical Survey of India, Southern Circle Coimbatore, Indi作檢驗,並由Department of Botany, Bharathiar University, Coimbatore, India保存。

    為了保存GLEt,會切碎500克的G montanum,並浸泡在1.5L的95%乙醇中一晚。

    懸浮液已經經過過濾處理,而殘留物則再次使其漂浮在相同容量的95%乙醇中,持續48小時,並過濾。

    兩份過濾議會合併,而容液會在rotavapor中以408C-508C的溫度低壓蒸發。

    墨綠色的殘留物會凍乾並至於8C至48C的溫度中儲存,以備後用。

    需要時,將殘留物懸浮於過濾液中使用。

    參考資料: me
    • 登入以對解答發表意見
還有問題?馬上發問,尋求解答。