蘋果 發問時間: 科學化學 · 1 0 年前

關於isotopic labeling technique

想請問一下

isotopic labeling technique這種技術

在化學上主要的目的及作法分別是什麼?

以及在化學上這種技術扮演了什麼樣的角色

感謝!!!!!

1 個解答

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  • 1 0 年前
    最佳解答

    1.同位素標幟追蹤技術,是在通過系統的樣品的化學組成中殖入特殊的同位素(例如: 在水中殖入O-18同位素),之後,在系統的某部份如果發現到此同位素,就表示必來自此樣品,由此可以推知此樣品在系統中通過的情形

    Isotopic labeling is a technique for tracking the passage of a sample of substance through a system. The substance is 'labeled' by including unusual isotopes in its chemical composition. If these unusual isotopes are later detected in a certain part of the system, they must have come from the labeled substance.

    2.被同位素標幟的化合物可以從質譜儀或紅外光譜儀偵測到

    There are two ways to detect the presence of labeling isotopes. Since isotopes have different masses, they can be separated using mass spectrometry. Another consequence of the difference in mass is that molecules containing isotopes have different vibrational modes; these can be detected by infrared spectroscopy.

    3.同位素標幟追蹤技術也可以用來研究化學反應機構,從同位素在被標幟的反應物和生成物中的分佈可以幫助化學家暸解反應如何進行

    Isotopic labeling can also be used to study chemical reactions. In this method specific atoms are replaced by an isotope in a reactant molecule which then participates in a chemical reaction. With spectroscopy, nuclear magnetic resonance spectroscopy for example, it is now possible to identify where a particular molecular fragment in the reactant ends up as a particular fragment in one of the reaction products.

    4.例如:

    將酚C6H5OH 加入含有以重氫標幟的重水(D2O)的水(H2O)中,結果我們可以從質譜或紅外光譜中測到被重氫標幟的酚(C6H5OD), 顯示出,在水溶液中,酚的-OH 基上的氫可以跟水分子的氫交換

    5.放射性同位素標幟追蹤技術Radioisotopic labelling

    以放射性同位素作為標幟同位素,可用放射線偵測技術偵測樣品或反應產物的分佈

    參考資料:

    http://en.wikipedia.org/wiki/Isotopic_labeling

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