zhong 發問時間: 社會與文化語言 · 1 0 年前

子宮頸癌的paper 有人能幫我翻譯嗎?(不要翻譯軟體的)

Abstract

Background: Endometrial cancer is the fourth most prominent cancer among all feminine cancers

in the Western world. Resveratrol, a natural anti-oxidant found in red wine emerging as a novel

anticancer agent, exerts antiproliferative and pro-apoptotic activity in various cancer cell types, but

its effect on uterine cancer cells is poorly understood. At the molecular level, resveratrol has been

reported to inhibit cyclooxygenase (COX) expression and/or activity; in endometrial cancer cells,

COX-2 is overexpressed and confers cellular resistance to apoptosis. The aim of the present study

was to determine if resveratrol could exert anti-proliferative and pro-apoptotic activity over

uterine cancer cells upon inhibition of COX-2 expression and/or activity. Six different human

uterine cancer cell lines were used as a model (HeLa, Hec-1A, KLE, RL95-2, Ishikawa and EN-

1078D).

Results and discussion: High-dose of resveratrol triggered apoptosis in five out of six uterine

cancer cell lines, as judged from Hoechst nuclear staining and effector caspase cleavage. In

accordance, uterine cancer cell proliferation was decreased. Resveratrol also reduced cellular

levels of the phosphorylated/active form of anti-apoptotic kinase AKT. Endogenous COX-2 protein

levels were decreased, concomitant with a decrease in production of COX metabolites PGE2 and

PGF2α, in each uterine cancer cell line expressing detectable levels of COX-1 and/or COX-2 in

presence of resveratrol. Although COX expression was identified as a target of resveratrol in

uterine cancer cells, inhibition of COX activity or exogenously added PGE2 did not modulate the

effect of resveratrol on cellular proliferation.

Conclusion: High-dose of resveratrol exerts tumoricidal activity over uterine cancer cells and

regulates COX expression. In these cells, resveratrol would not directly target COX activity, but

possibly other enzymes involved in prostaglandin synthesis that act downstream of the COXs.

1 個解答

評分
  • jason
    Lv 5
    1 0 年前
    最佳解答

    摘要

    背景介紹:子宮內膜癌是西方女性癌症的第四位。白藜蘆醇(Resveratrol),一種紅酒裡面的天然抗氧化物,由於具有抗細胞增生以及加速細胞凋零的作用,因此可用來對抗癌症。但白藜蘆醇對字宮癌細胞的作用還是不清楚。在分子層面來看,白藜蘆醇可以抑制環氧合酶(cyclooxygenase COX )的表現及活性,而子宮內膜癌細胞的COX-2活性有過度表現的情形,因此使得細胞無法自然凋零(而一直持續成長而成為癌細胞)。本實驗的目的在於驗證白藜蘆醇是否可以經由抑制COX-2的活性而發揮抗細胞增生作用與促進細胞凋零。六株不同的人類子宮癌細胞株用來當實驗的標本。這六種細胞株的名稱是: (HeLa, Hec-1A, KLE, RL95-2, Ishikawa and EN-1078D).

    結果與討論:利用Hoechst核染色技術以及caspase蛋白酵素切割活力,可以發現高劑量的白藜蘆醇在六種細胞株中誘發其中的五種細胞株產生細胞凋零,同時子宮癌細胞的分裂也減少了。白藜蘆醇也減少了具有活性的AKT(抗細胞凋零激脢)的量。在可以偵測出COX的細胞裡,細胞內的COX-2都降低了,從而使得COX的代謝物PGE2以及PGF2α也減少了。雖然白藜蘆醇的作用目標是COX,但是,直接抑制COX的活性或加入PGE2來減少COX被抑制,卻都不會改變白藜蘆醇對抗細胞增生的作用

    結論:高劑量的白藜蘆醇具有殺死子宮癌細胞及抑制COX這種蛋白脢表現的作用。但是白藜蘆醇不是直接作用於COX,而可能是經由COX下游的化學反應,這些化學反應都與前列腺素的合成酵素有關。

還有問題?馬上發問,尋求解答。