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Hair follicle organ culture

↑以上為段落標題

Human scalp hair follicles were isolated and cultured in vitro, as described previously. Briefly, dissected hair follicles were cut into small pieces of approximately 2.5 mm in length from the bottom of dermal papillae and cultured in 24-well dishes for 10 days in Williams E medium containing 10ng/ml hydrocortisone, 10μg/ml insulin, 2mM L-glutamine, 100μg/ml streptomycin, and 100U/ml penicillin at 37℃ in a 5% CO2 atmosphere. EGCG was added to culture media at 0.1,1,or5μM. In all experiments culture media and EGCG were changed every other day. A total of 30 anagen hair follicles from 3 different volunteers (10 follicles per subject) were cultured under each growth condition, respectively. The values shown are means±SEM of triplicate cultures.

以上為段落內容~麻煩盡可能口語話一些...

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  • Lucky
    Lv 6
    1 0 年前
    最佳解答

    題目:頭皮毛囊組織培養

    Human scalp hair follicles were isolated and cultured in vitro, as described previously.

    在前段已描述人類頭皮毛囊是由體外培養分離出來

    Briefly, dissected hair follicles were cut into small pieces of approximately 2.5 mm in length from the bottom of dermal papillae and cultured in 24-well dishes for 10 days in Williams E medium containing 10ng/ml hydrocortisone, 10μg/ml insulin, 2mM L-glutamine, 100μg/ml streptomycin, and 100U/ml penicillin at 37℃ in a 5% CO2 atmosphere.

    簡單的說,將頭皮毛乳頭細胞分離出來的毛囊切成約2.5 mm小片段使用Williams E培養基,培養基含有10ng/ml hydrocortisone(氫可體松), 10μg/ml insulin(胰島素), 2mM L-glutamine (麩胺醯胺), 100μg/ml streptomycin(鏈黴素)及100U/ml penicillin(盤尼西林或青黴素)的24個well(孔洞)的碟子培養在37℃ 含有 5% CO2 溫箱的環境中10天

    EGCG was added to culture media at 0.1,1,or5μM.

    兒茶素可在培養基可加0.1,1或5μM的濃度

    In all experiments culture media and EGCG were changed every other day.

    在所有培養經驗,兒茶素需隔天更換

    A total of 30 anagen hair follicles from 3 different volunteers (10 follicles per subject) were cultured under each growth condition, respectively.

    在所有30生長期毛囊是取自3位不同自願者(每個人取10個毛囊)分別培養在單一的生長環境中

    The values shown are meansSEM of triplicate cultures.

    3組培養數值以算數平均數測量標準誤差(standard error of measurement, SEM)表示

    參考資料: 研究助理
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