[英文]關於生物科技文章-英文翻譯 3

[Opposite effects of oxytocin on proliferation of osteosarcoma cell lines]

2.3. Measurements of cell proliferation

The effects of oxytocin on cell proliferation were measured by [3H]

thymidine incorporation (measures DNA synthesis) and the EZ4U-kit

(which is based on the capability of living cells to reduce slightly or

uncoloured tetrazolium salts into intensely coloured formazon

derivates). SaOS-2, TE-85 and UMR-106 cells were detached with

trypsin-EDTA and seeded in: 96 well plates for the EZ4U kit (2000

cells per well) and in 24 well plates for [3H]thymidine incorporation

(5000–10,000 cells per well) in McCoy's 5A medium containing 10%

FBS, 50 IU/l penicillin and 50 µg/ml streptomycin or in α-MEM

containing 10% FBS, 50 IU/l penicillin, 50 µg/ml streptomycin and

2 mM L-glutamine. The cells were grown for 48–72 h. The 96 well

plates were then cultivated for 24 h in serum free Dulbecco's medium

without phenol red with 0.1% BSA and 2 mM 1-glutamine, and the 24

well plates in serum free McCoy's 5A medium with 0.1% BSA or α-

MEM with 0.1% BSA. The cells were thereafter incubated with various

concentrations of oxytocin, the oxytocin antagonist or oxytocin

together with the oxytocin antagonist for 24 or 48 h.

The concentration of the oxytocin antagonist was based on earlier

doseresponse studies and previous publications (see for example).

Proliferation of cells in the 96 well plates were analyzed by the EZ4U

kit. Cells in the 24well plateswere pulsedwith [3H]thymidine

[1 µCi/ml] during the last 2 h.

Thereafter the cells were rinsed twicewith 1 ml ice cold 0.9% NaCl and then left for 15 min in 5% ice cold trichloroacetic acid.

Precipitated material was extracted with 0.5 ml of 0.1 mol/l NaOH for 2 h at room temperature under constant shaking.

The radioactivity in eachwell wasmeasured in a liquid scintillation β-counter after addition of 4 ml Ultima Gold scintillation liquid.

2 個解答

評分
  • 1 0 年前
    最佳解答

    [在催產素對面的作用在osteosarcoma細胞系的擴散]

    2.3. 細胞增殖的測量

    催產素的作用在細胞增殖測量由[3H]

    胸腺嘧啶脫氧核苷並網(措施脫氧核糖核酸綜合)和EZ4U成套工具

    (根據活細胞的能力輕微地減少或

    uncoloured tetrazolium鹽到強烈地色的formazon裡

    衍生物)。 SaOS-2、TE-85和UMR-106細胞分開了與

    胰蛋白乙二胺四乙酸和播種在: 96塊好的板材為EZ4U成套工具(2000年

    細胞每口井)和在24塊好的板材為[3H]胸腺嘧啶脫氧核苷並網

    (每口井5000個¡ V10,000細胞)在McCoy的包含10%的5A媒介

    FBS、50 IU/l青黴素和50 µg/ml肺病特效藥或者在£ \ - MEM

    包含10% FBS, 50 IU/l青黴素, 50 µg/ml肺病特效藥和

    2毫米L谷氨酰胺。 細胞為48 ¡ V72 h.增長。 96井

    板材為24 h任意然後耕種了在清液Dulbecco的媒介

    沒有苯酚磺酞以0.1% BSA和2毫米1谷氨酰胺和24

    好的板材在清液釋放McCoy的5A媒介與0.1% BSA或£ \ -

    MEM與0.1% BSA。 細胞爾後孵化了與各種各樣

    催產素、催產素反對者或者催產素的集中

    與催產素反對者一起為24或48 h。

    催產素反對者的集中及早根據

    doseresponse研究和早先出版物(例如參見)。

    在96塊好的板材EZ4U分析細胞的擴散

    成套工具。 細胞在24well plateswere pulsedwith [3H]胸腺嘧啶脫氧核苷

    [1 µCi/ml]在最後2 h.期間。

    爾後細胞是被漂洗的twicewith 1機器語言冰冷的0.9% NaCl然後左在15分鐘在5%冰冷的三氯乙酸。

    被沉澱的材料提取了以0.5機器語言0.1 mol/l NaOH為2 h在室溫在恆定震動之下。

    放射線在eachwell在一液體發出火花£ wasmeasured] -逆在4機器語言的加法以後Ultima金子發出火花液體。

    參考資料: word
  • 匿名使用者
    6 年前

    你厭倦往返補習班浪費的時間和體力嗎

    你想在家裡躺在沙發,就可以上課嗎?

    網路課程橫跨升學考試、教職考試、公職考試、執照考試、就業考試、語言進修等6大類50餘科,

    超過500多門課程,為各界人士提供優質的線上教育服務。

    自100年6月成立以來,使用會員在一年內就已突破3萬名,並培育出許多優秀的學員,

    學員踴躍分享行動補習帶來的便利與效果,累積許多佳績見證。

    免費試讀網址:

    http://ad.mobchannels.com/redirect.phpk=b2d8a7d711...

還有問題?馬上發問,尋求解答。