生物科技翻專業英文翻譯~請高手幫忙翻譯

Topic- [Opposite effects of oxytocin on proliferation of osteosarcoma cell lines]

4. Discussion

However, it has one disadvantage since also cells which not are viable

at the time of measurement are registered. Therefore we used the

commercially available kit EZ4U as a complement. This kit is based on

the capability of living cells to reduce slightly coloured or uncoloured

tetrazolium salts into intensely coloured formazon derivates. The two

methods gave almost similar results, except that also lower concentrations of oxytocinwere efficient when analyzed by the EZ4U-kit. The

reason behind this discrepancy might be that the experimental groups

(number of wells) in the experiments analyzed by the EZ4U kit were

larger and thereby easier could reach statistical significance.

We have in previous studies found that oxytocin stimulates

proliferation of human osteoblast-like cells and SaOS-2 cells. In

contrast, oxytocin has also been reported to inhibit proliferation of

some other cell lines including some osteosarcoma cell lines [19].

Therefore we investigated the effects of oxytocin on cell proliferation

in two human osteosarcoma cell lines known to represent different

stages of osteoblast differentiation. The human osteosarcoma cell line

SaOS-2 is rather highly differentiated and expresses both osteocalcin

and ALP which are well-known osteoblastic markers [20].

Fig. 1. Proliferation of SaOS-2 cells incubated for 24 h with oxytocin measured by: A. the EZ4U-kit (oxytocin 1–100 pmol/l n=20 for each concentration, oxytocin 1000 pmol/l n=15, control n=18) and B–C. [3H]thymidine incorporation (B: oxytocin 10–1000 pmol/l n=5 for each concentration, control n=5); (C: oxytocin 100 pmol/l n=5, oxytocin 100 pmol/l together with the oxytocin antagonist 200 pmol/l n=5, control n=10). The results are expressed as percentage of the control (0) and shown as means±SD.

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    主題[對面的影響催產素對擴散的骨肉瘤細胞系]

    4.討論

    但是,它有一個缺點,因為還沒有細胞是可行的

    在當時的測量登記。因此,我們使用了

    商用套件EZ4U作為一種補充。這種試劑盒是基於

    能力的活細胞,以減少或略有黃色uncoloured

    四鹽到激烈的塗上formazon衍生物。這兩個

    方法了幾乎相同的結果,但也低濃度的有效oxytocinwere時分析了EZ4U包。那個

    原因這一差異可能是實驗組

    (人數井)在實驗中分析了EZ4U包被

    較大,從而更容易達到統計學意義。

    我們已經在以前的研究發現,催產素刺激

    增殖的成骨樣細胞和SaOS - 2細胞。印第安納州

    相比之下,催產素也已報告給抑制

    其他一些細胞系包括一些骨肉瘤細胞系[ 19 ] 。

    因此,我們調查的影響催產素對細胞增殖

    在兩個人成骨肉瘤細胞株已知來代表不同的

    階段的成骨細胞分化。人類骨肉瘤細胞系

    SaOS - 2是相當高度分化的,並表示雙方骨鈣

    和ALP這是眾所周知的成骨細胞標記[ 20 ] 。

    圖 .1 .擴散SaOS - 2細胞培養24小時催產素衡量如下: A.在EZ4U傑(催產素1-100 pmol /的ln = 20為每個濃度,催產素1000個pmol /的ln = 15 ,控制18例)和B - C上升。 [小時] TdR摻入( B組:催產素10到1000 pmol /的ln = 5的每個濃度,控制5例) ; (丙:催產素100 pmol /的ln = 5 ,催產素100 pmol / L的一道催產素受體拮抗劑200 pmol /的ln = 5 ,控制10例) 。結果是百分

    比表示的控制( 0 ) ,並顯示為手段±標準差。

    ................願耶穌基督保祐..................

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