慈媽咪 發問時間: 科學生物學 · 1 0 年前

peptone 及 tryptone的差異

請問一下 ,

1.Peptone 跟 Tryptone 都叫蛋白胴 是一樣的東西嗎?

2.LB medium是用tryptone,YEP medium則是用peptone,請問兩者可亦互換使用嗎?

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1 個解答

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  • 1 0 年前
    最佳解答

    Tryptone:

    Tryptone is the assortment of peptides formed by the digestion of casein by the protease trypsin.

    Tryptone is commonly used in microbiology to produce Lysogeny broth for the growth of E. coli and other microorganisms. It provides a source of amino acids for the growing bacteria.

    Peptone:

    Salmon Protein Hydrolysate is a high quality source of peptides and free amino acids. The product is manufactured by controlled enzymatic hydrolysis of salmon tissue. Peptone from is well suited for the growth of Bacillius, Escherichia, yeasts etc.The effectivity can be proved with addition of yeast extract.

    兩種含不同的養分( peptides or free amino acids),不建議互換

    參考資料: Wiki and Sigma
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