? 發問時間: 社會與文化語言 · 1 0 年前

化學的報告,非常《急》,請善心及專業人士幫忙一下!20點

以下是原文報告的一部份,

這是文章的PDF檔

http://www.jstage.jst.go.jp/article/bpb/28/2/323/_...

我只想知道文章裡重點在哪裡,如果分成"摘要"‧"實驗"‧"結論" 三部分去報告的話該怎麼分配?

如果有人能幫我解答,真的非常感謝!

4,4-Dihydroxybiphenyl as a New Potent Tyrosinase Inhibitor

The color of mammalian skin is determined by many factors, for which visible ones are the degree and distribution

of melanin pigmentation. Because tyrosinase, (polyphenol oxidase) is the key enzyme for melanin

biosynthesis, the use of various tyrosinase inhibitors is a common practice for whitening purpose in cosmetics. In

the present study, the inhibition of tyrosinase by 4,4-dihydroxybiphenyl (44-BP) was investigated. In addition to

tyrosinase inhibiting activity, melanin biosynthesis was assessed in B16F10 melanoma cells (B16 cells). The results

showed that 44-BP exhibits a strong anti-tyrosinase activity with IC501.91mM. The kinetic analysis of tyrosinase

inhibition revealed that 44-BP acts a competitive inhibitor (Ki4.0104M at 2.5mM and Ki2.1

105M at 5mM). Furthermore, data on melanin biosynthesis indicated that the amount of melanin was clearly

suppressed by 44-BP.

3 個解答

評分
  • John
    Lv 6
    1 0 年前
    最佳解答

    4,4-Dihydroxybiphenyl as a New Potent Tyrosinase Inhibitor

    Abstract:

    1) The degree and distribution of melanin pigmentation is one of many factors to determine the color of mammalian skin. Its reason is what tyrosinase is the key enzyme for melanin biosynthesis.

    2) A common practice for whitening purpose in cosmetics is to use various tyrosinase inhibitors

    Investigation (or assessment) in the present study:

    1) the inhibition of tyrosinase by 4,4-dihydroxybiphenyl (44-BP)

    2) melanin biosynthesis in B16F10 melanoma cells (B16 cells) to tyrosinase inhibiting activity

    Conclusion:

    1) The results shown: 44-BP exhibits a strong anti-tyrosinase activity with IC501.91mM.

    2) The kinetic analysis of tyrosinase inhibition revealed: 44-BP acts a competitive inhibitor (Ki4.0104M at 2.5mM and Ki2.1 105M at 5mM)

    3) The data on melanin biosynthesis indicated: the amount of melanin was clearly

    suppressed by 44-BP.

    2008-12-16 09:10:53 補充:

    4,4- Dihydroxybiphenyl as a New Potent Tyrosinase Inhibitor

    以『4.4-二羥基聯苯』作為一種新高效能的『酪氨酸酶抑制劑』

    2008-12-16 09:11:21 補充:

    Abstract: 摘要

    1) The degree and distribution of melanin pigmentation is one of many factors to determine the color of mammalian skin. Its reason is what tyrosinase is the key enzyme for melanin biosynthesis. 黑色素沉積的散佈與程度,對於決定哺乳動物膚色許多因素中的一項,其理由是:對於黑色素生物合成而言,『酪氨酸酶』是個主要的『酶』。

    2008-12-16 09:12:08 補充:

    2) A common practice for whitening purpose in cosmetics is to use various tyrosinase inhibitors.對於化妝品美白目的,一個普遍作法,就是使用不同的『酪氨酸酶抑制劑』。

    2008-12-16 09:12:44 補充:

    Investigation (or assessment) in the present study: 現行研究的調查(或評估)

    1) the inhibition of tyrosinase by 4,4-dihydroxybiphenyl (44-BP) was investigated.藉由『44-二羥基聯苯』的『酪氨酸酶』抑制效能是被調查的。

    2008-12-16 09:14:09 補充:

    2) melanin biosynthesis was assessed in B16F10 melanoma cells (B16 cells) to tyrosinase inhibiting activity.對於『酪氨酸酶』的抑制活性,在B16F10黑色素瘤細胞中,黑色素生物合成是被評估的。

    2008-12-16 09:14:29 補充:

    Conclusion: 結論

    1) The results shown: 44-BP exhibits a strong anti- tyrosinase activity with IC501.91mM. 其結果顯示:『44-二羥基聯苯』呈現出強大抑制『酪氨酸酶之IC501.91mM活性』。

    2008-12-16 09:14:49 補充:

    2) The kinetic analysis of tyrosinase inhibition revealed: 44-BP acts a competitive inhibitor (Ki4.0104M at 2.5mM and Ki2.1 105M at 5mM) 『酪氨酸酶』抑制的動力學分析顯現:『44-二羥基聯苯』扮演著一個有競爭力的抑制劑。

    2008-12-16 09:15:17 補充:

    3) The data on melanin biosynthesis indicated: the amount of melanin was clearly

    suppressed by 44-BP. 黑色素生物合成資料指出:『44-二羥基聯苯』明確地抑制黑色素總量。

    2008-12-18 10:29:25 補充:

    我只能從這篇文章說一說我的見解:

    1.目的:驗證『二羥基聯苯』是一種新的且高效能的『酪氨酸酶』抑制劑

    說明:

    1.1)黑色素沉積的散佈與程度,對於決定哺乳動物膚色許多因素中的一項,其理由是:就黑色素生物合成而言,『酪氨酸酶』是個關鍵的『酶』。

    1.2)對於化妝品美白目的,一個普遍作法,就是使用不同的『酪氨酸酶』抑制劑。

    2008-12-18 10:29:50 補充:

    2.實驗:

    A.調查『二羥基聯苯』對『酪氨酸酶』抑制效能 (其結果如何?要靠你們自己實驗)

    B.針對『酪氨酸酶』抑制活性,評估在B16F10 黑色素瘤細胞中的黑色素生物合成(其結果如何?要靠你們自己實驗)

    2008-12-18 10:30:20 補充:

    3.結論:(文章說的,或許你們的實驗結果與文章說的結論不同)

    3.1『二羥基聯苯』呈現出強大抑制『酪氨酸酶之IC501.91mM活性』。←就實驗結果。

    3.2『二羥基聯苯』扮演著一個有競爭力的抑制劑。←就『酪氨酸酶』抑制的動力學分析。

    3.3『二羥基聯苯』明確地抑制黑色素總量。← 就黑色素生物合成數據指出。

    參考資料: myself, myself, myself, myself, myself, myself, myself, myself, myself
  • 1 0 年前

    不好意思...呵呵

    我就是太嫩了,所以不知道重點 = "摘要"

    不過我還有一點不太明白...

    可以請大師幫我解答嗎?

    回答中的"現行研究的調查(或評估)"

    就是教授要我們做的實驗步驟與結果嗎?

    因為其他組都會以自己的文章加以分段報告

    我們如果是3的人要分3段part

    中間實驗,我想更清楚了解一點,

    sorry...

    因為我有點急...

    所以迫切想知道更清楚的解釋

    謝謝你囉^^

    2008-12-18 03:49:27 補充:

    剛剛看清楚你的評論了...

    是要分成,"目的"‧"實驗"‧"結論"

    那篇文章對我來說真的很深澳....

    如果大師你覺得不麻煩的話,

    可以跟我在解釋一下...

    怎麼樣去把那篇文章分成那三段去報告

    然後細節怎麼報告...

    才不會被教授打槍嗎?

    拜託你了!!

  • 1 0 年前

    重點 = "摘要"。 分成"目的"‧"實驗"‧"結論" 三部分去報告。

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