Adam翔 發問時間: 社會與文化語言 · 1 0 年前

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The aim of this study was to use gene therapy via the Sleeping Beauty (SB) system to increase telomerase promoter activity to target hepatocellular carcinoma (HCC). In previous studies, we identified selective and increased expression of luciferase and suicide genes controlled by the hTERT (human telomerase reverse transcriptase) promoter and the SV40 enhancer in telomerase-positive cancer cell lines. Because telomerase is activated in about 80% of HCCs, it is likely that increasing the activity of the telomerase promoter with a suicide gene will effectively eradicate HCCs. We found that the telomerase promoter mediated SB system can efficiently insert transgene into HCC genomes. Also, telomerase promoter activity was increased using a SB vector expressing suicide gene HSV-TK (herpes simplex virus thymidine kinase) controlled by the hTERT promoter and a SV40 enhancer for the induction of telomerase-positive cancer-specific cell death. HCC cell lines transfected with pT.hTp.HSV-tk.Con with active helper plasmid and ganciclovir (GCV) significantly inhibited cancer cell growth. These results indicate that Sleeping Beauty transposon mediated suicide gene expression can be used in HCC-targeted cancer gene therapy.

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  • 1 0 年前
    最佳解答

    HALLO!!

    讓me幫您吧!

    這項研究的目的是要用於通過臥美 (SB) 系統的基因治療增加端粒酶啟動子活動為目標肝癌 (肝癌)。 在以前的研究,我們發現選擇性和增加基因表達的螢光素酶及自殺案受 hTERT (端粒酶逆轉) 發起人和端粒酶陽性癌細胞 SV40 增強。 因為在約 80%的 HCCs 中啟動端粒酶,可能是,增加端粒酶發起人自殺基因的活動將有效地消除 HCCs。 我們發現端粒酶啟動子介導 SB 系統可以有效地插入轉基因肝癌基因組。 此外,端粒酶啟動子活動增加使用表達自殺基因 HSV-TK (單純皰疹病毒胸苷激酶) 由 hTERT 發起人和端粒酶陽性癌症特定細胞死亡的感應的 SV40 增強控制 SB 載體。 染與活動傭工粒 pT.hTp.HSV tk.Con 肝癌細胞和更 (GCV) 昔洛韋顯著抑制癌細胞生長。 這些結果顯示可以在肝癌針對癌基因治療使用睡美人轉座子介導自殺基因表達。

    參考一下喲!

    參考資料: Microsoft® Translator
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