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Genetic material can be introduced into chromosomes

of all organisms via viruses or transposons. Sleeping

Beauty (SB) transposon system, a type of mobile

element that belongs to the Tc1/mariner class of

transposons, is a promising tool for gene delivery due

to its ability to express therapeutic genes stably as well

as to infect resting cells.1,2) At specific TA sequences,

SB moves in a simple, cut-and-paste manner. Although

popular tools of gene therapy, viral vectors are not ideal

due to immunogenic problems, potential RCV (Repli-

cation Competent Virus) outbreaks, and difficulties in

mass production. The advantages of the Sleeping Beauty

transposon system include prolonged transgene expres-

sion without elicitation of an immunogenic response, no

possibility of RCV, and ease of construction. SB is an

excellent candidate for cancer gene therapy because it

promotes long-lasting gene expression even in mitoti-

cally active tissues such as tumors, but also lacks many

of the drawbacks related to viral vectors.3,4)

Telomerase is a ribonucleoprotein complex the

function of which is to add telomeric repeats to

chromosomal ends. It consists of two essential compo-

nents, the telomerase RNA template (hTR) and the

catalytic subunit (hTERT). The hTERT promoter is

activated only in cells and tissues with telomerase

activity, i.e., tumor or stem cells.5,6) Because telomerase

is activated in 80–90% of hepatocellular carcinomas

(HCCs),7) it is an ideal candidate for anti HCC cancer

therapy. The aim of this study was to determine the

effects of the HSV-TK gene under the control of the

hTERT promoter with the SV40 enhancer delivered via

SB on hepatocellular carcinogenesis. SB vector system

which contains suicide gene HSV-TK controlled by the

hTERTp and SV40 enhancer was constructed in order to

suppress tumor cell growth and induce apoptosis in

hepatocellular carcinoma cell lines. The results of this

study indicate that the hTERT promoter and SV40

enhancer delivered via the SB are effective in targeted

cancer gene therapy.

1 個解答

  • 1 0 年前





    病毒或轉座子通過的所有生物。 睡

    美 (SB) 轉座子系統,一類型的移動

    屬於的 Tc1/水手類的元素



    感染休息 cells.1,2) 在 specific 電訊管理局局長序列,

    SB 移動以一種簡單、 剪切和粘貼的方式。 雖然


    由於到潛在 RCV (Repli-的免疫原性問題

    陽離子能力的病毒) 的暴發和在 difficulties

    大量生產。 臥美的優點



    RCV 和建設易用性的可能性。 理事會是一個


    促進長效基因表達甚至 mitoti-

    cally 活動組織如腫瘤,但也沒有很多

    缺點與有關病毒性 vectors.3,4)

    端粒酶是複雜的 ribonucleoprotein,


    染色體結束。 It consists of 兩個基本有限公司-

    指數,端粒酶 RNA 範本 (hTR),

    催化亞單位 (hTERT)。 hTERT 發起人是


    活動,即,腫瘤或幹 cells.5,6) 因為端粒酶

    在肝癌 80–90%中啟動

    (HCCs) 7) 它是一個理想的抗肝癌癌症的候選人

    治療。 這項研究的目的是要確定,

    控制下的 HSV-TK 基因 effects,

    通過傳遞 SV40 增強與 hTERT 啟動子

    對肝細胞癌的理事會。 SB 向量系統

    它包含由 HSV-TK 控制的自殺基因,

    hTERTp 和 SV40 增強建造,以


    肝癌細胞系。 這結果

    研究顯示,SV40 與 hTERT 啟動子

    通過在理事會的增強是 effective 的目標



    2009-05-11 18:04:34 補充:

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    參考資料: Microsoft® Translator