被動免疫和保護測試的文章翻譯

The experimental protocols were reviewed and approved by the

Hokkaido University Animal Care and Use Committee (08-0234).

Six-week-old female BALB/c mice were passively immunized by

intraperitoneal injection with 200 mg of purified MAb S139/1 or

ZGP12/1.1 (IgG2a) [18] in 0.5 ml of PBS. Twenty four hours

before or after immunization, mice were challenged intranasally

with 50 ml of 10650% mouse infectious dose of Aichi (H3) or

WSN (H1) under anesthesia with isoflurane. Three days after the

challenge, mice were euthanized to obtain the lung tissue samples.

The lung homogenates (10% w/v) prepared in MEM were

disrupted and centrifuged at 3,0006g for 10 min, and then the

supernatants were examined for virus infectivity. Virus titers were

measured by a plaque assay using MDCK cells.

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    實驗協議進行審查和批准

    北海道大學動物護理和使用委員會(08-0234)。

    六週齡雌性BALB / c小鼠的被動免疫

    腹腔注射200毫克的純化抗體S139 / 1或

    ZGP12/1.1(IgG2a的)[18]在0.5毫升的PBS。 24個小時

    免疫之前或之後,小鼠鼻腔挑戰

    50毫升10650%小鼠感染劑量的愛知縣(H3的)或

    無線傳感器網絡(上半年)異氟醚麻醉下。三天後,

    挑戰,小鼠安樂死,以獲取肺組織樣本。

    肺組織勻漿(10%瓦特/五)編寫的培養基是

    干擾和離心3,0006克10分鐘,然後

    上清審查了病毒的傳染性。病毒滴度

    衡量一空斑測定MDCK細胞使用。

    參考資料: 我+Google翻譯 :D
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