The experimental protocols were reviewed and approved by the
Hokkaido University Animal Care and Use Committee (08-0234).
Six-week-old female BALB/c mice were passively immunized by
intraperitoneal injection with 200 mg of purified MAb S139/1 or
ZGP12/1.1 (IgG2a)  in 0.5 ml of PBS. Twenty four hours
before or after immunization, mice were challenged intranasally
with 50 ml of 10650% mouse infectious dose of Aichi (H3) or
WSN (H1) under anesthesia with isoflurane. Three days after the
challenge, mice were euthanized to obtain the lung tissue samples.
The lung homogenates (10% w/v) prepared in MEM were
disrupted and centrifuged at 3,0006g for 10 min, and then the
supernatants were examined for virus infectivity. Virus titers were
measured by a plaque assay using MDCK cells.
- 1 0 年前最佳解答
六週齡雌性BALB / c小鼠的被動免疫
腹腔注射200毫克的純化抗體S139 / 1或
衡量一空斑測定MDCK細胞使用。參考資料： 我+Google翻譯 :D