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顗喆 發問時間: 社會與文化語言 · 1 0 年前


Genotyping bacterial isolates with various methods is frequently

used to compare the genetic relatedness of bacterial

strains and provides useful information for

epidemiological studies. In a previous study, we used emm

(gene of M protein) sequencing [5], vir typing [6] and

pulsed-field gel electrophoresis (PFGE) typing to analyze

a collection of streptococcal isolates from scarlet fever

patients and used these data to build a DNA fingerprint

and emm sequence database for long-term disease surveillance

[7]. Vir typing has since been abandoned in our lab

because it has lower discriminatory power than PFGE and

the protocol is difficult to standardize with conventional

agarose gel electrophoresis. In contrast, the PFGE protocol

for S. pyogenes has been standardized in our laboratory,

and a second enzyme, SgrAI, has been found to replace

SmaI for analysis of strains with DNA resistant to SmaI

digestion [7]. Since PFGE is highly discriminative and

emm sequencing provides unambiguous sequence information

regarding emm type, we adopted these two genotyping

methods to characterize streptococcal isolates and

build a Streptococcus pyogenes DNA fingerprint and

sequence database for the long-term study of scarlet fever

and other streptococcal diseases.



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