In conclusion, an SYBR Green-Based real-time PCR assay was
established for monitoring and quantification of B. ostreae in the gill
tissue of oysters. This assay was shown to be sensitive and specific
and, moreover, was able to generate reproducible results.
Comparison showed a very good correlation between heart imprint and the real-time PCR techniques
Thus this assay may be used in
the future for detection and monitoring bonamiosis in various
tissues of infected oyster and may be adapted for parasite detection
in the environment to improve the knowledge of the parasite life
cycle within and outside of the host.