yumi 發問時間: 社會與文化語言 · 10 年前

急!!醫學期刊翻譯<與微物抗藥有關>6

Southern blot hybridization with probes specific for armA, rmtB, and blaCTX. The

isolates used for hybridization were randomly selected from among the isolates from

different regions and hospitals that had different plasmid digestion profiles. The

EcoRI-digested plasmids from armA and rmtB carriers were hybridized with probes

specific for blaCTX, armA, and rmtB. Southern blot hybridization was performed

with a digoxigenin labeling and detection kit (Roche, Mannheim, Germany), as

recommended by the manufacturer. The probes specific for armA, rmtB, and

blaCTX-M were obtained by PCR amplification with the primer pairs listed in Table 1.

PFGE analysis. In order to characterize the clonality of all

armA- and rmtB-positive strains, we performed molecular typing.

Forty-four armA-positive isolates were all typeable by

PFGE. PFGE analysis revealed that four small clusters of

isolates were clonally related (Fig. 2). The first cluster comprised

five strains (type A), and the second cluster comprised

two strains (type B), with both types being isolated from the

same hospital in the western region. The third cluster comprised

three strains (type C) from two hospitals, and the fourth

cluster comprised two strains (type D) from one hospital in the

southern region. The other 32 armA-positive isolates had dis-tinct PFGE patterns. Of the 37 rmtB-positive strains, 34 isolates

were typeable by PFGE and 3 were nontypeable. Four

small clusters of isolates were clonally related (Fig. 3). The first

cluster (type I) and the second cluster (type II) comprised two

strains each from two different hospitals in the northern region.

The third cluster comprised five strains (type III), and the

fourth cluster comprised two strains (type IV); all seven strains

in the third and fourth clusters were from the same hospital in

the western region. The other 23 isolates had distinct PFGE

patterns.

2 個解答

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  • 10 年前
    最佳解答

    Southern印跡雜交探針具體的武裝突襲,rmtB和blaCTX。該

    菌株用於雜交,隨機選取其中株

    不同地區和醫院,有不同的質粒消化配置文件。該

    經 EcoRI酶切質粒載體從 ARMA及rmtB與探針進行雜交

    具體的blaCTX,武裝突襲,並 rmtB。 Southern印跡雜交進行

    與地高辛標記和檢測試劑盒(羅氏,曼海姆,德國),作為

    製造商建議。探針具體的武裝突襲,rmtB,和

    blaCTX - M的,得到了PCR擴增引物對同列於表1。

    電泳分析。為了表徵所有的克隆

    武裝突襲和rmtB陽性菌株,我們進行分子分型。

    四十四個武裝突襲陽性菌株均typeable由

    電泳。電泳分析表明,四個小集群

    菌株DNA複製有關(圖 2)。第一組包括

    5株(A型),而第二組組成

    二株(B型),有兩種類型被隔離

    同一家醫院在西部地區。第三組包括

    三株(C型)由兩所醫院,第四

    集群包括兩個品系(D型)在從一個醫院

    南部地區。其他32名武裝突襲的陽性菌株有存款保險計劃,tinct電泳模式。在37 rmtB陽性菌株,34株

    通過電泳進行 typeable不可分型3例。四

    小群的菌株進行 DNA複製相關(圖 3)。第一

    集群(I型)和第二組(第二類)包括兩名

    株分別來自兩個不同的醫院在北部地區。

    第三組包括5株(III型),以及

    第四組包括兩個株(IV型),所有7個菌株

    在第三和第四團簇來自同一醫院

    西部地區。其餘 23個菌株具有明顯的電泳

    模式。

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