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Question

Two microliters of the restriction enzyme digestion mixture was mixed with 0.5 μl of MapMarker 1000 size standard and 12 μl of deionized formamide, followed by denaturation at 96°C for 2min and immediate chilling on ice.

這一段英文我遇到瓶頸了.....

Answer 1

最佳解答

2μl restriction enzyme digestion mixture 消化酵素混合液

+ 0.5 μl of MapMarker 1000 size standard

+12 μl of deionized formamide 去離子性福馬林

followed by denaturation at 置於96°C for 2min

and immediate chilling on ice 後立即使用冰塊冷卻

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Answer 2

Is this another way of blanching?

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Answer 3

Where did you hit the wall? It looks straightforward to me.

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