專討遇到瓶頸

Question

Two microliters of the restriction enzyme digestion mixture was mixed with 0.5 μl of MapMarker 1000 size standard and 12 μl of deionized formamide, followed by denaturation at 96°C for 2min and immediate chilling on ice.

這一段英文我遇到瓶頸了.....

Answer 1

最佳解答

2μl restriction enzyme digestion mixture 消化酵素混合液

+ 0.5 μl of MapMarker 1000 size standard

+12 μl of deionized formamide 去離子性福馬林

followed by denaturation at 置於96°C for 2min

and immediate chilling on ice 後立即使用冰塊冷卻

Answer 2

Is this another way of blanching?

Answer 3

Where did you hit the wall? It looks straightforward to me.