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For the hydroponic trial, complete nutrient solutions were prepared with 0.25 mmol L−1 KH2PO4,0.2 mmol L−1 CaCl2, 0.5 mmol L−1 MgSO4, 50 μmol L−1 Fe-EDTA, 50 μmol L−1 KCl, 25 μmol L−1H3BO3, 2 μmol L−1 MnSO4, 2 μmol L−1 ZnSO4, 0.5 μmol L−1 CuSO4, and 0.5 μmol L−1 Na2MoO4. There were five N treatments imposed by... 顯示更多 For the hydroponic trial, complete nutrient solutions were prepared with 0.25 mmol L−1 KH2PO4,0.2 mmol L−1 CaCl2, 0.5 mmol L−1 MgSO4, 50 μmol L−1 Fe-EDTA, 50 μmol L−1 KCl, 25 μmol L−1H3BO3, 2 μmol L−1 MnSO4, 2 μmol L−1 ZnSO4, 0.5 μmol L−1 CuSO4, and 0.5 μmol L−1 Na2MoO4.
There were five N treatments imposed by supplying N at 3 mmol L−1, including sole NH+4 , sole NO−3 and in combinations of both N forms at NH+4 /NO−3 ratios of 70/30, 50/50, and 30/70. NaNO3 and (NH4)2SO4 were used as the N sources of NO−3 and NH+4 , respectively, except in the 50/50 treatment,where NH4NO3 was used. Seeds were germinated in sand with saturated CaSO4 solution in an incubator at 25 ◦C. When the first leaf expanded (about 8 d), uniform oat seedlings were removed from sand carefully, rinsed with distilled water, and transplanted to 10-L tanks with aerated nutrient solutions. Each tank was fitted with a 6-hole cover that held 3 plants in each hole (18 plants in a tank). Everyday, 1 mmol L−1 KOH or HCl was used to readjust the pH of nutrient solution to 6.5. Nutrient solutions were changed every 3 d. Each treatment was established in 3 replications (3 tanks). Three replicate plants were harvested after 15, 30, and 45 d.

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