病毒學論文, 跪求翻譯(拜託別使用google之類翻譯軟體)

專有名詞可以不用翻, 主要是文法太爛, 所以跪求翻譯

Among the Ebola viruses most species cause severe hemorrhagic fever in humans; however, Reston ebolavirus (REBOV) has not been associated with human disease despite numerous documented infections. While the molecular basis for this difference remains unclear, in vitro evidence has suggested a role for the glycoprotein (GP) as a major filovirus pathogenicity factor, but direct evidence for such a role in the context of virus infection has been notably lacking. In order to assess the role of GP in EBOV virulence, we have developed a novel reverse genetics system for REBOV, which we report here.

Together with a previously published full-length clone for Zaire ebolavirus (ZEBOV), this provides a unique possibility to directly investigate the role of an entire filovirus protein in pathogenesis. To this end we have generated recombinant ZEBOV (rZEBOV) and REBOV (rREBOV), as well as chimeric viruses in which the glycoproteins from these two virus species have been exchanged (rZEBOV-RGP and rREBOV-ZGP). All of these viruses could be rescued and the chimeras replicated with kinetics similar to their parent virus in tissue culture, indicating that the exchange of GP in these chimeric viruses is well tolerated. However, in a mouse model of infection rZEBOV-RGP demonstrated markedly decreased lethality and prolonged time to death when compared to rZEBOV, confirming that GP does indeed contribute to the full expression of virulence by ZEBOV. In contrast, rREBOV-ZGP did not show any signs of virulence, and was in fact slightly attenuated compared to rREBOV, demonstrating that GP alone is not sufficient to confer a lethal phenotype or exacerbate disease in this model. Thus, while these findings provide direct evidence that GP contributes

已更新項目:

to filovirus virulence in vivo, they also clearly indicate

that other factors are needed for the acquisition of full virulence.

2 個已更新項目:

可以給你整個paper嗎?

2 個解答

評分
  • ?
    Lv 6
    8 年前
    最佳解答

    首先伊波拉病毒家族中全部的病毒都會造成出血性發燒

    除了這個以外 --> Reston ebola virus (REBOV)

    試管測試證實病毒毒性跟glycoprotein (GP)有關

    不過不能證明直接關聯

    所以為了測試

    他們把Reston ebola virus的GP基因跟另外一個會致死的Zaire ebola virus(ZEBOV)換

    做了該做的測試證實交換的基因確實有被表現

    在老鼠實驗中

    發現交換過的(ZEBOV)的致死率變低了

    老鼠多活了幾天

    證明GP跟病毒表現有關

    不過因為REBOV沒有變成致死

    能做的結論只有GP跟致死性有關不過不是唯一的因素

    還有其他未知的原因

    大概是這樣

    有需要更深入再問吧(雖然說不一定知道)

    話說這只是abstract吧

    整個paper呢?

  • 7 年前

    到下面的網址看看吧

    ▶▶http://*****/

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