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Alternative transcription of the same gene in sense and antisense orientation regulates expression of protein-coding genes.

Here we show that noncoding RNA (ncRNA) in sense and antisense orientation also controls transcription of rRNA genes

(rDNA). rDNA exists in two types of chromatin—a euchromatic conformation that is permissive to transcription and a hete-

rochromatic conformation that is transcriptionally silent. Silencing of rDNA is mediated by NoRC, a chromatin-remodeling

complex that triggers heterochromatin formation. NoRC function requires RNA that is complementary to the rDNA promoter

(pRNA). pRNA forms a DNA:RNA triplex with a regulatory element in the rDNA promoter, and this triplex structure is rec-

ognized by DNMT3b. The results imply that triplex-mediated targeting of DNMT3b to specific sequences may be a common

pathway in epigenetic regulation. We also show that rDNA is transcribed in antisense orientation. The level of antisense RNA

(asRNA) is down-regulated in cancer cells and up-regulated in senescent cells. Ectopic asRNA triggers trimethylation of

histone H4 at lysine 20 (H4K20me3), suggesting that antisense transcripts guide the histone methyltransferase Suv4-20 to

rDNA. The results reveal that noncoding RNAs in sense and antisense orientation are important determinants of the epigenetic

state of rDNA.

2 個解答

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  • 7 年前
    最佳解答

    Alternative transcription ofthe same gene in sense and antisense orientation regulates expression ofprotein-coding genes.相同基因的可變轉錄在sense股 和antisense 股用來調控蛋白質編碼基因的表現。(當轉錄進行時,雙股DNA中會被RNApolymerase當作模板的一股即為sense strand,與sense strand互補的一股則為antisense strand)

    Here we show thatnoncoding RNA (ncRNA) in sense and antisense orientation also controlstranscription of rRNA genes

    (rDNA).我們發現非編碼RNA(ncRNA)在sense股 和antisense 股也會控制rRNA基因的轉錄。 rDNA exists in two types of chromatin—aeuchromatic conformation that is permissive to transcription and a hete-

    rochromaticconformation that is transcriptionally silent.rDNA存在於兩種染色質中-同染色質結構(允許轉錄)和異染色質(不進行轉錄)。 Silencing of rDNA is mediated by NoRC, achromatin-remodeling

    complex thattriggers heterochromatin formation.rDNA的靜默現象是由NoRC(一種染色體重建複合體用來觸發異染色質)調控著。 NoRC function requires RNA that iscomplementary to the rDNA promoter (pRNA).NoRC啟動需要RNA補充到rDNA啟動子(pRNA)。 pRNA forms a DNA:RNA triplex with a regulatoryelement in the rDNA promoter, and this triplex structure is recognized byDNMT3b.pRNA 形成DNA:RNA與調控單元在rDNA啟動子中形成三股合成,這個三股結構會被DNMT3b所認出。 The results imply that triplex-mediatedtargeting of DNMT3b to specific sequences may be a common

    pathway inepigenetic regulation.這個結果意味三股結構會被DNMT3b認為特定序列標靶,這可能是表關基因調控的一般路徑。 We also show that rDNA is transcribed inantisense orientation. The level of antisense RNA

    (asRNA) isdown-regulated in cancer cells and up-regulated in senescent cells.我們也知道rDNA會被antisense股轉錄。asRNA能夠用來降低癌細胞的敏感度,提升老化細胞的活性。 Ectopic asRNA triggers trimethylation of histone H4 at lysine 20(H4K20me3), suggesting that antisense transcripts guide the histonemethyltransferase Suv4-20 to rDNA.異位asRNA 會觸發 H4組蛋白在H4K20me3的甲基化,意味著反義轉錄會導引著組蛋白轉錄抑制因子Suv4-20到rDNA The results reveal that noncoding RNAs insense and antisense orientation are important determinants of the epigeneticstate of rDNA.這樣的結果顯示著非編碼RNAs在正反兩股轉錄占了重要的角色,可以決定rDNA的表關基因化程度。

    2014-04-15 16:03:12 補充:

    可以參考我的翻譯http://yuhang0522.pixnet.net/blog/category/4335023

  • 匿名使用者
    7 年前

    參考下面的網址看看

    http://phi008780414.pixnet.net/blog

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